Effect of Quercetin on the Expression of NOXs and P-Smad3C in TGF-Β-Activated Hepatic Stellate Cell Line LX-2

Background: Hepatic stellate cells (HSCs) play a primary role in liver fibrogenesis. NOXs are the main origin of reactive oxygen species (ROS) liver. Among them, NOX1, NOX2, and NOX4 expressed more HSCs involved development fibrosis. Quercetin, an abundant citrus flavonoid, is known to have beneficial effects on injury hepatic Objectives: In this study, effect quercetin expression Smad3 phosphorylation induced by TGF-β human LX2 cell line was investigated. Methods: The cytotoxic were determined MTT assay. activated with 2 ng/mL for 24 h then treated different concentrations Quercetin. mRNA rates NOX4, phosphorylated Smad 3C (p-Smad3C) analyzed using real-time polymerase chain reaction (PCR) western blot assays. Results: increased protein level p-Smad3C line. Quercetin significantly decreased LX-2 cells. Moreover, diminished TGF-β. Conclusions: may be effective improving fibrosis via reduction HSCs. mechanism through which reduces these target genes related level.